Increasing fluorescence lifetime for resolution improvement in stimulated emission depletion nanoscopy
نویسندگان
چکیده
منابع مشابه
Supercontinuum stimulated emission depletion fluorescence lifetime imaging.
Supercontinuum (SC) stimulated emission depletion (STED) fluorescence lifetime imaging is demonstrated by using time-correlated single-photon counting (TCSPC) detection. The spatial resolution of the developed STED instrument was measured by imaging monodispersed 40-nm fluorescent beads and then determining their fwhm, and was 36 ± 9 and 40 ± 10 nm in the X and Y coordinates, respectively. The ...
متن کاملPlasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy.
Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved nanoscopy. We demonstrate stimulated emission depletion (STED) nanoscop...
متن کاملNanoparticle-assisted stimulated-emission-depletion nanoscopy.
We show that metal nanoparticles can be used to improve the performance of super-resolution fluorescence nanoscopes based on stimulated-emission-depletion (STED). Compared with a standard STED nanoscope, we show theoretically a resolution improvement by more than an order of magnitude, or equivalently, depletion intensity reductions by more than 2 orders of magnitude and an even stronger photos...
متن کاملFluorescence Nanoscopy of Single DNA Molecules by Using Stimulated Emission Depletion (STED)**
Lens-based (far-field) fluorescence microscopy has played a key role in the life sciences, but for most of the time the resolution has been limited to about Dr= l/(2NA)> 200 nm, with l denoting the wavelength of light and NA the numerical aperture of the lens. However, since the 1990s microscopy concepts have emerged providing diffraction-unlimited resolution by inhibiting the fluorescence of t...
متن کاملDefining the Noise-limited-resolution for Stimulated Emission Depletion Microscopy
With recent developments in microscopy, such as stimulated emission deletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we analyse how the interplay between shot noise, thermal noise, the saturation intensity of a fluorescent label, and the damage threshold of the sample changes the maximum resolution of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biophotonics
سال: 2019
ISSN: 1864-063X,1864-0648
DOI: 10.1002/jbio.201800315